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The images from immunostaining were exported as Leica image files and were processed using LASX software
( a ) Fasting plasma GIP (CON n = 11, Gipr AP-KO n = 12), ( b ) total GLP-1 (CON n = 11, Gipr AP-KO n = 12) ( c ) insulin (CON n = 11, Gipr AP-KO n = 12), ( d ) food intake post IPGTT, ( e ) ad lib fed blood glucose, ( f ) RER, ( g ) energy expenditure (inset, with ANCOVA using BW as covariate), ( h ) ambulatory activity (x beam breaks) of std chow fed (lean) mice ( d - h : CON n = 11, Gipr AP-KO n = 13, symbols as defined in f)
It highlighted gaps in long-term data beyond 612 months and heterogeneity in dosing protocols [5][26]
Campbell JE, Mller TD, Finan B, DiMarchi RD, Tschp MH, DAlessio DA
