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2.6 Quantitative PCR (qPCR) analysis Total RNA was extracted from the fresh liver tissues by EZ-press RNA Purification Kit (Cat#B0004DP, EZBiocience, Roseville, CA, United States), Color Reverse Transcription Kit (Cat#A0010CGQ, EZBiocience, Roseville, CA, United States) was used to convert mRNA to cDNA, and 2 * Color SYBR Green qPCR Master Mix (Cat#A0012-R2, EZBiocience, Roseville, CA, United States) was used for qPCR under the standard procedure

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