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The hAMY 3 R cells were cultured in growth medium consisting of DMEM (Gibco, #31966-021) supplemented with 10% v/v FBS (Gibco, #16140-071), 1% v/v Penicillin-Streptomycin (Gibco, #15140-122), 0.5 mg/mL Geneticin G418 (Gibco, #10131-027), 0.4 mg/mL Hygromycin (Invitrogen, #10687010) and 250 nM Methotrexate (Sigma, A6770)
The liver stores B12, and these stores can last a significant time (up to several years for non-deficient individuals), but when they are depleted, it takes consistent input to rebuild and maintain them
Warning : Consult your healthcare professional before use if pregnant, nursing, taking medications, or for any use by minors
Dopamine metabolism can generate reactive molecules, while the substantia nigra naturally contains abundant iron
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