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Early investigations in diverse occupational groups, including oil rig workers, telecommunications employees, and nurses, utilized 24-hour urine collections to compare melatonin output between DSW and NSW, often revealing reduced melatonin levels during NS or rotating shift schedules 172,173,174
In 2025, an independent group at Brunel University London reported dose-dependent telomere length extension in normal human cells, the first solid Western replication of that claim
At 6 hours at room temperature, estimated potency loss is 5-10%
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