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Inflammatory markers, while they do tend to rise with weight regain, may not return to their pre-treatment peaks if the regain is partial rather than complete
Such an event could lead to serious health complications
RNA yield and integrity were initially assessed from absorbance readings at 260 and 280 nm using a Nanodrop 1000 spectrophotometer (Thermo Fisher Scientific, Loughborough, UK), and confirmed using an Agilent Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent Technologies, Wokingham, UK) according to the manufacturers guidelines
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