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(1) SDT fatty rats given certified diet-experimental animal diet 2 (CE-2), a standard chow provided from CLEA (CLEA, Tokyo, Japan) (DKD-vehicle), (2) SDT fatty rats given CE-2 containing empagliflozin (EMPA)(30 mg/kg, Boehringer-Ingelheim, Germany)for 12 weeks (DKD-EMPA), and (3) SDT fatty rats given empagliflozin diet for first 6 weeks and switched to CE-2 containing EMPA and linagliptin (LINA) (3 mg/kg, Boehringer-Ingelheim, Germany) (DKD-EMPA + LINA)

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These include the peptides quantity, the target concentration required for the study, and the precision needed for laboratory measurements