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Freezing tirzepatide permanently damages the formulation and makes it unsafe or ineffective
After removing the supernatant, cells were washed with PBS, fixed with 4% paraformaldehyde for 15 min, stained with DAPI for 5 min, and visualized under a confocal laser scanning microscope (LSCM
The RF imputation was executed with 5 multiple datasets (m = 5), 20 iterations, and 200 trees per forest
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