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Mice underwent isoflurane anesthesia in the induction chamber (2% isoflurane at 0.5 L/min O 2 ) and then were shaved from the neckline to mid-chest level
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In the case of rAmy:AMY 3 R:G s , the histidine residues predicted as tautomer were: H226 (CTR), H41, H357 (G), 54, 183, 311 (G)
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