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The pellet was dissolved in 90 ml Buffer B supplemented with 500 mM KCl and 5 mM Imidazole and incubated with 2 ml TALON resin (Takara, Cat#635502) for 1 h on a rotary mixer
Generally well tolerated, with injection-site reactions the most common adverse event
No FDA-approved human trial
Since the net charge of CAND is -0.2, resulting in an overall neutral charge, it is difficult for CAND to be effectively transported to the cell nucleus on its own
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