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Distilled samples were diluted 1:300 in ddH 2 O and analyzed on a liquid water isotope analyzer (Los Gatos Research, Los Gatos, CA, USA) against a standard curve prepared with samples containing different concentrations of D 2 O [23, 25]

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GCL is a heterodimeric enzyme composed of a glutamate-cysteine ligase catalytic subunit (GCLC), which confers enzymatic activity, and a glutamate-cysteine ligase modifier subunit (GCLM), which enhances GCLC efficiency and attenuates feedback inhibition by GSH

doi:10.1016/S0968-0004(00)01712-6