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An acid is used to create a controlled burn on the skin
Isolated RNA was purified using DNase I (Sigma) before conversion to cDNA using the GoScript Reverse Transcription system (Promega)
Methylation of the CDH1 and VIM genes in epithelial ovarian cancer (EOC) can be detected using MS-HRM and is a reliable and sensitive method for detecting promoter methylation and early detection of EOC (Thakur et al., 2019)
doi: 10.1038/s41467-019-12384-2 48 RichardLCHuiWenL
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