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Lastly, it is important to look at how other anti-diabetic therapies affect HF to select an appropriate drug regimen for patients with excessive risk factors or established HF
Successful isolation of monocytes was confirmed with flow cytometry (BD Fortessa) using VivaFix Viability Assay (BioRad), a cocktail of antibodies from BioLegend: anti-CD3 PE (300456), anti-CD14 BV650 (301835), anti-CD19 PerCP-Cy5.5 (302229), and anti-CD56 APC (318309)
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