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The primary risks include amplified gastrointestinal side effects (nausea, vomiting), potential hypoglycaemia in patients with diabetes taking certain medications, dehydration from combined fluid loss, and interference with weight management goals due to alcohol's caloric content
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The method uses a gradient of solvents with an initial ratio of 10:90 (v/v, aqueous: organic phase) at a flow rate of 1 mL/min linearly changing to 90:10 over 10 min and kept constant for one minute
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