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For two-step reactions, an initial reaction was performed in 90% of the final reaction volume with all components present except for GSH and GST
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This led to the discovery of NP-BTA, a N-pyridinyl--thienyl-acrylamide derivative, was discovered to inhibit the essential enzyme glutaminyl-tRNA synthetase (Gln4), representing a novel antifungal mechanism of action (Fu et al., 2021)
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