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Indeed 5-HT 2C agonists appear to regulate DA neurotransmission by decreasing DA cell activity in the VTA, a region also rich in GHSR1a expression (Di Giovanni et al., 2000)
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The resulting lysates were incubated with either magnetic beads conjugated to anti-Flag antibodies or protein A/G agarose beads coupled to the corresponding antibodies (Thermo Fisher Scientific, 88802) at 4 C for 3 h
DrMR: And I think one of the things that elude many patients is when we say start, the definition of that can vary wildly from person to person
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