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This approach follows general peptide cycling logic and is a reasonable precaution given limited long-term human data
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Diet, microbiome and symptom context: Diet and microbiome factors can be relevant in selected patients, but they should be considered alongside clinical assessment rather than used as a replacement for evidence based IBD care
Chromatography was performed on a Kinetex 2.6 m HILIC 100 , 100 2.1 mm internal diameter column (Phenomenex), with a constant flow rate of 250 l/min and a solvent gradient from 20% Mobile Phase B (50:45:5, ACN:H 2 O:100 mM AmFm in H 2 O) to 22% B at 5 min, 40% B at 10 min, 60% B at 13 min, 80% B at 15 min, and 99% B at 20 min
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