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In vitro cAMP assay For GLP-1R agonist activity, CHOK1 cells stably expressing human GLP-1R were used to measure peptideantibody conjugate-induced cAMP production in a homogenous time-resolved fluorescence assay (Cisbio, cat

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Plan for behavioral and dietary changes in the months following discontinuation, since appetite typically returns to baseline

Wende AR, Schell JC, Ha CM, Pepin ME, Khalimonchuk O, Schwertz H, Pereira RO, Brahma MK, Tuinei J, Contreras-Ferrat A, Wang L, Andrizzi CA, Olsen CD, Bradley WE, DellItalia LJ, Dillmann WH, Litwin SE, Abel ED (2020) Maintaining myocardial glucose utilization in diabetic cardiomyopathy accelerates mitochondrial dysfunction