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The effects of a single treatment can last several days in your system, which is why treatments are usually spaced out rather than done daily

10.1371/journal.pone.0106890 5 Al-ZamelN.Al-SabahS.LuqmaniY.AdiL.ChackoS.SchneiderT

Aside from C1QBP, insulin/IGF-1 can also alter the nuclear factor-kB (NF-kB) signal pathway, Polo-like kinase 1 (PLK1), NADPH oxidase 4 (NOX4), and mammalian target of rapamycin (mTOR) to reverse apoptosis, repair DNA and induce multi-drug resistance gene (Lee et al., 2007