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Fluorescence intensities were quantified using ImageJ software, and the red-to-green fluorescence ratio was calculated to determine mitochondrial polarization

Treatment is subject to candidacy, health history, allergies, medications, pregnancy status, and appropriate expectations

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For a 5mg vial: Add 2.5ml bacteriostatic water Resulting concentration: 2mg/ml (2000mcg/ml) For a 100mcg dose: draw 0.05ml (5 units on a standard insulin syringe) For a 200mcg dose: draw 0.10ml (10 units) For a 10mg vial: Add 3.0ml bacteriostatic water Resulting concentration: 3.33mg/ml (3333mcg/ml) For a 100mcg dose: draw 0.03ml (3 units) For a 200mcg dose: draw 0.06ml (6 units) Alternatively, for easier math with a 5mg vial, you can add 5ml of bacteriostatic water to create a 1mg/ml (1000mcg/ml) concentration