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This involved adding a lipid-ethanol solution to a plasmid solution in 25 mM citrate buffer (pH 4.0), followed by buffer replacement with PBS through diafiltration using Amicon Ultra Centrifuga Filter (10 kDa MWCO, Millipore)
Accessed 10/25/2022, Rabinowitz PM
The peptides will still bind to their receptors, but the downstream cascade they're designed to trigger gets suppressed at multiple points in the pathway
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