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Anticipating that wild-type mice would exhibit increased susceptibility and a more pronounced response to a higher APAP dose, AAV-transduced wild-type mice were treated with 600 mg/kg APAP for subsequent analysis

Instead of mimicking gut hormones, phentermine stimulates the release of norepinephrine in the brain a neurotransmitter that suppresses appetite and increases alertness and energy

Cell transfection The shVRK2-expressing and VRK2-overexpressing plasmids were purchased from Genechem (Shanghai, China) and then used for constitutive expression of shRNA or cDNAs