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We have previously reported that GRK2 levels increase in several key metabolic tissues in experimental conditions promoting insulin resistance (IR) and that GRK2+/ mice (the use of GRK2/ animals is not experimentally possible since they die during embryogenesis [33]) are protected against high-fat diet (HFD)-induced obesity and IR

The cv showed a slope of 4.95 A M 1 , corresponding to an area-normalized sensitivity of 99 A M 1 cm 2 , with excellent linearity (R 2 = 0.99) and a LOD of 4.29 M, indicating that cv is most suitable for mechanistic investigations rather than trace-level detection