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In a cohort study, individuals who progressed to Alzheimers showed elevated 3-HAA, higher 3-HAA/anthranilic acid ratio, increased 3-HK, and reduced PA, suggesting these KP changes could serve as early biomarkers of cognitive decline [249]

Ewing et al.21 found many macrophages with rich pigment deposition on the mucosal lamina propria of the colon, the colonic submucosa and the submucosal lymphatic vessels through hematoxylin and eosin (HE) staining, which could also be discovered in the colonic mucosa and lymph nodes by periodic acid-Schiff staining (PAS), melanin staining, Fite staining and Congo red staining.22 Gomori iron staining is negative in the colonic mucosa and lymph nodes, as is the immunohistochemistry results for S100, keratin and HMB-45

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