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Diary cards were provided to participants on days 1, 7, 14, and 21, onto which participants were instructed to record their temperature, adverse events (AEs), medication, and supplementation
Rabbit polyclonal antibody against HIF-1 was obtained from (Novus Biologicals, Littleton, CO, USA, #NB100449), while phosphorylated mTOR S2448, -actin, -Tubulin, as well as Glycolysis Antibody Sampler Kit to detect Pfkp, Pkm2, Pkm 1/2, Hexokinase 1, Hexokinase 2 and Pdh were all obtained from (Cell Signaling Technology, Beverly, MA, USA, #2971, #4970, #2148, #8337 respectively)
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