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Concentration (start) Volume (start) = Concentration (final) Volume (final) In Vivo : Select the appropriate dissolution method based on your experimental animal and administration route

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After 2 days of growth, colonies were counted and harvested in subpools of 75 000 100 000 and frozen in PCR tubes at OD 600 2.0 4.0 (~100 million CFU mL 1 , or ~100 CFU per mutant) at -80 C