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For cells, they were first washed with PBS and fixed at room temperature for 15 min, followed by incubation with the staining solution at pH 6.0 overnight at 37 C
Harvard Medical School
While historically listed with an "unknown status," recent data releases in late 2025 and early 2026 have clarified the safety profile that helped justify the Category 1 reclassification. Study Design: A randomized, placebo-controlled, double-blind Phase 1 pilot study
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