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Eight hours later, old medium was replaced with Williams E medium (without phenol red) supplemented with maintenance supplement kit B (Dexamethasone + Penicillin/Streptomycin + Human Insulin + Transferrin + Selenium Complex + Bovine Serum Albumine + Lenoleic Acid + Glutamax + HEPES buffer) (Gibco/Invitrogen, USA)

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