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Notably, significant abnormalities are observed in pathways such as galactose metabolism, steroid biosynthesis, and bile acid biosynthesis. Post-treatment with metformin, MAFLD rats demonstrated notable changes in certain metabolic pathways, particularly in PPAR signaling and fat digestion and absorption. This suggests that metformin may regulate these specific metabolic pathways, aiding in restoring normal metabolic functions or at least alleviating metabolic dysregulation

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Tafazoli and OBrien, 2009) and in vivo (Shimizu et al., 2009), the exact cellular mechanisms and pathways of AQ-QI and DEAQ-QI toxicity remain poorly understood