The debenzylated product was precipitated in hexane, dried using a centrifuge and vacuum, dissolved in basic water, and purified by dialysis with a dialysis membrane (molecular weight cut-off 3.5 kDa) against deionized water (DIW) for three days before the lyophilization for removing water and collecting the final product
As this process repeats, the area may begin to look darker or more uneven in tone
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For tendon, ligament, and joint repair, subcutaneous injection near the target tissue produces significantly stronger local effects than oral administration