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Hydrochloric acid or sodium hydroxide may be added to adjust pH
The binding of insulin with IR causes autophosphorylation of IR [20, 21], activating downstream phosphoinositide-3-kinase (PI3-K)/Akt and mitogen activated protein kinase (MAPK) pathways, which affect the differentiation, energy metabolism and activity of osteocytes [22, 23]
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doi: 10.1016/j.bbagen.2012.10.005 96 GargS
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