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After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions

Figure 2d shows the load-displacement curve of polystyrene substrate measured by an in-situ nanomechanical test system

These results suggest that ER36 is able to upregulate ALDH1A1 expression in breast cancer cells by responding to the agonist activity of tamoxifen

Amongst these mutations, 182 (83.9%) are single nucleotide substitutions, 19 (8.7%) are multiple nucleotide substitutions, 11 (5.1%) are deletions, and 5 (2.3%) are intronic mutations