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Spinal stenosis (neurogenic claudication) typically causes leg pain, weakness, or numbness that starts after standing or walking for a while

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To solubilize and alkali-extract the samples, homogenates were centrifuged at 21,000 g for 5 min at room temperature and the pellet of each sample was resuspended in an equal volume (200 l) of 3% (w/v) of sodium dodecyl sulfate (SDS)

A year later Gerhard Heinrich, a senior researcher in Habener's laboratory, reported with Ira Wilson and other colleagues the successful cloning and sequencing of a rat pancreatic preproglucagon