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The absence of established diagnostic biomarkers or an international gold standard for DILI frequently results in diagnostic delays [55,56,57]
Hydration is non-negotiable: Drink water consistently throughout the day, even if you do not feel thirsty
In particular, dedicated outcome trials have shown that SGLT-2Is significantly slow CKD progression and reduce adverse kidney-related outcomes in patients with CKD regardless of diabetes status [5,6,7]
We initiated an evolution campaign with seven PANCE passages on GD_M, followed by 12 passages of high-stringency dual selection penalizing proteases that cleave VAMP1, and concluded with 16 passages on a circuit using a weaker promoter in front of the GD_M substrate to increase positive selection stringency (Extended Data Fig
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