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Whole-cell patch-clamp recordings were performed on mRFP--actin-transfected hippocampal neurons to measure miniature excitatory postsynaptic currents (mEPSCs), reflecting synaptic activity [2]

The D-amino acid substitution preserves the spatial orientation of amino acid side chains needed for competitive binding to the FOXO4-p53 interaction surface, maintaining biological activity despite the non-natural backbone (PMID: 28340339)

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