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Gene expression levels were standardized using the z score method, and cluster analysis of DEGs in key pathways was performed using the pheatmap R package [33] based on standardized FPKM values

Clinical trial data shows increased incidence of biliary disease, likely related to rapid weight loss and altered bile composition during metabolic changes

For example, one study found that taking 1,0002,000 micrograms orally in properly timed doses helped improve levels as much as injections