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The fragment library for the vBrood application was prepared using the CHOMP application to fragment the ChEMBL database 77 version CHEMBL25 78
Inhibition of 5-LOX in a Cell-Free Assay The enzyme activity of human recombinant 5-LOX was determined spectrophotometrically by monitoring the oxidation of H 2 DCFDA to the highly fluorescent 2,7-dichlorofluorescein during the catalytic reaction mediated by 5-LOX as reported (Pufahl et al., 2007)
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