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Beads were sequentially washed in 500-l solutions of: 1x 1% SDS in 50 mM HEPES, 1x 500 l 0.2% SDS in 50 mM HEPES, 1x 500 l 0.2% SDS, 40 mM CAA, 10 mM TCEP in 50 mM HEPES (10-min incubation with agitation) and finally 2x 500 l 50 mM HEPES
Its generally safe to combine fibre supplements with gentle osmotic laxatives, but always space them apart by at least 2 hours and consult your healthcare provider for personalised advice.
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