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Protein extracts were separated on 8% SDS-polyacrylamide or 412% bistris protein gel (Invitrogen), transferred on a nitrocellulose membrane, and stained with antibodies against ZO-1 (N1N2, GeneTex, dilution 1:3000)
Reconstitution by the researcher also allows control over the final solution concentration
There is need for long-term prospective trials of GLP-1 drugs to confirm efficacy in breast cancer prevention
This can make it harder to digest fats
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