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On the day of the assay, serial dilutions (7-fold dilutions, 7 concentrations per compound and one well containing only assay buffer) of test compounds were performed in duplicate in assay buffer to appropriate concentrations in 96-well plates (Greiner, U-shape #GR-650201) using a Biomek i7 liquid handler
Accordingly, researchers should evaluate claims or statements regarding GHK-Cus safety with due caution
If you take metformin regularly, talk to your health professionals about getting screened for vitamin B12 deficiency
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