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Initial studies demonstrated that although mature erythrocytes contain procaspase-3 and procaspase-8, they are devoid of the mitochondrial apoptotic machinery, e.g., APAF-1, cytochrome c, and caspases-2, -6, -7, and -9 [22, 23]
The "body protection compound" name reflects this protective function discovered in early research
After washing in PBS/BSA, the O/PEs were mounted on slides using Vectashield (H-1000-10, Vector Laboratories, Newark, CA, USA)
The number of needle punctures increases contamination risk over time
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