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The primary outcomes were the percentage weight change and the proportion of participants losing at least 5% of their baseline weight, using treatment-policy estimand analysis (intention-to-treat principle)

The 177191 fragment was identified as the lipolytic active site, and a tyrosine residue was added at the N-terminus to enhance stability

METHOD DETAILS Sample preparation FOXO4 FHD (95-195) was subcloned into the pET His6 Tobacco Etch Virus (TEV) LIC cloning vector (2B-T, obtained from Scott Gradia, Addgene Plasmid #29666) and transformed into BL21 (DE3) cells

No bad effects, normal blood work, and the drug cleared from the blood within 24 hours