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Subjects Members of the mitochondrial solute carrier (SLC) family 25 (SLC25) provide transport steps for substances across the mitochondrial inner membrane into the mitochondria that are needed for biochemical pathways and cellular homoeostasis
For experiments in 384-well plates, wells were aspirated, rinsed with PBS and incubated with a fixative and staining solution containing 3.7% formaldehyde (Fisher Scientific, F75P-1GAL) and 5 g ml 1 Hoechst 33342, trihydrochloride, trihydrate (Invitrogen, H1399)
The pathway enrichment was performed by metaboanalyst ( In addition, we randomly split all samples into 70% training set and 30% testing set to assess the overall performance of the model