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reported an investigation from Germany that utilized a randomized, placebo-controlled, triple-blind trial using confocal laser scanning microscopy to determine the cosmetic effects and tolerance of a commercial drinkable collagen supplement (2.5 g with vitamins C and E plus minerals [zinc and biotin]) for 12 weeks in 60 female participants 4070 years of age [32]
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Briefly, cells were seeded onto 24-well chamber slides, rinsed with ice-cold PBS and methanol at 20 C for 10 min, and then permeabilized with 0.1% Triton X-100
2% (v/v) 2-mercaptoethanol, 4% (w/v) SDS, 50 mM Tris (pH 7.0), 10% (v/v) glycerol, 0.02% Coomassie Brilliant Blue R-250) and incubated at 65 C for 10 min
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