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Samples were separated using a Kinetex 2.6 m XB-C18 100 , 100 2.1 mm LC Column (Phenomenex) with a constant flow rate of 250 l/min following a flow gradient starting at 70% Mobile Phase B (ACN + 0.5% Acetic acid, AA) then going up to 99% for 5 min before re-equilibration at 70% for 5 min

Human tri peptide GHK-Cu is an anti-aging peptide that is known to enhance collagen production, which is not only beneficial for the skin but also for wound healing and other bodily functions