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Excessive Ca 2+ entry into the cytosol leads to pericyte calcium overload and contraction, causing microvascular constriction

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To isolate chondrocytes, fresh articular cartilage tissues were rinsed with basal medium (BM), consisted of high glucose Dulbeccos modified Eagles medium (DMEM, Gibco/Thermo Fisher Scientific, Waltham, MA, United States), with 2 Antibiotics-Antimycotics (anti-anti, Life Technologies, Carlsbad, CA, United States) and then cut into 1 mm 3 pieces

Gene-Level Repair Signaling (JAK-2 / Egr-1): Turns on genes involved in cell survival and growth factor production essentially flipping the bodys repair switch at the DNA level