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After its made in the skin as vitamin D3 (cholecalciferol), it becomes converted by the liver and kidney into a secosteroid hormone (calcitriol) that has receptors in practically all our tissues
This was followed by three pairs of monopolar needles stabbed 3 mm deep into the tail at measurements of 10, 60, and 100 mm caudal to the base of the tail
Blood was centrifuged in a standard centrifuge (Labofuge 300, Heraeus Holding, Germany) at 3000 g 10 min
Rhonda Patrick adds: "NAD+ precursors like NMN increase levels, aiding DNA repair and energykey for anti-aging."[34] She suggests stacking with NNMT inhibitors for sustained effects
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