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DatLab 7.4 (OROBOROS Instruments, Innsbruck, Austria) was used for analysis

Our systematic characterization of semaglutides signalling mechanisms in the hindbrain reveals the intracellular signalling architecture through which semaglutide engages cAMP and calcium to regulate body weight, providing avenues for improving obesity therapeutics

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We next built a machine learning model to enhance prediction and further selected from the aforementioned features with less than 30% missing datapoints to build a prediction model for GLP-1 M responsiveness