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cDNA was synthesized using M-MLV Reverse Transcriptase (Invitrogen) and Random Primers (Invitrogen)
The resulting mixture was purified by column chromatography using silica gel and a mixture of ethyl acetate/hexane (1:10) as the mobile phase (Coates 2006)
Also assess for relevant co-morbidities as this may influence medicine selection and the approach to long-term management
To reduce potential toxicity, the positively charged groups on their surface are often modified into neutral or negatively charged components
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