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The fungal inoculum was prepared using axenic fungal cell cultures grown for 5 days, from which 1 mL of the fungal suspension was pelleted through centrifugation and was re-suspended in BG11 medium, which was then used as the inoculum
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The cells were washed with PBS and incubated with FITC-tagged exendin 939 dissolved in cold PBS at a concentration of 10 M for 30 min at 4 C
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