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The release of the nitrobenzoic acid group as nitro-2-thiobenzoate anion (TNB ) creates a yellow anion that can be measured spectrophotometrically at a wavelength of 412 nm
Reference: Ioanna A
Glutathione competitively inhibits melanin synthesis in the reaction of tyrosinase and L-DOPA by interrupting L-DOPAs ability to bind to tyrosinase during melanin synthesis
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